RNAConnect, developer of enzymatic tools for RNA visualization and manipulation, today announced the release of UltraMarathonRT® Two-step RT-qPCR kit, a next-generation tool for RNA researchers conducting gene expression analysis.
The UltraMarathonRT® The RT-qPCR kit dramatically improves data reliability in quantitative PCR by maintaining consistent cycle threshold (Ct) values, regardless of RT primer type, RNA complexity, or amplicon location. This innovation enables researchers to confidently quantify RNA in a wide range of applications, including biomarker validation, high-GC RNA analysis, transcriptional studies, and more.
UltraMarathonRT (uMRT) was designed to overcome the challenges of complex RNA secondary structures and low RNA inputs. Because uMRT provides complete 5′-3′ RNA transcript coverage, the location of the PCR amplicon along a first-strand cDNA molecule synthesized by uMRT does not affect qPCR quantification. This greatly reduces the potential for Ct value bias and/or variability seen when using non-editing retroviral RTs.
Many researchers are not even aware that existing RT-qPCR assays can provide very different results depending on the choice of primers. The innovative RT-qPCR based on UltraMarathonRT accurately quantifies gene expression regardless of primer selection, removing this element of variability to provide reliable data that researchers can trust.“
Ryan Muldoon, CEO, RNAConnect
UltraMarathonRT Key Features® RT-qPCR kit:
- Unrivaled data reliability: Provides consistent Ct values regardless of RT primer type, RNA complexity, or amplicon location, setting a new standard for accurate quantification.
- Enhanced sensitivity and efficiency: Detects RNA in a dynamic range of 0.1 pg to 2 µg, ideal for low-abundance RNA.
- High inhibitor tolerance: Works reliably in the presence of common blocking compounds such as ethanol, TRIzol and formalin.
- Improved workflow: Complete RT-qPCR experiments in less than an hour, accelerating research timelines.
- Preserving RNA integrity: UltraMarathonRT works optimally at ambient temperatures, eliminating the need for high-temperature steps and ensuring the integrity of RNA templates.
The UltraMarathonRT® The two-step RT-qPCR kit is also the ideal companion for validating novel genes detected in RNA-seq datasets, offering compatibility with RNA templates of all types, including long and non-coding RNAs.
The kit is available in three convenient sizes (200, 500 and 1,000 reactions) and volumes to meet various research needs.
